Multi-Disciplinary Approaches for Precision Delivery of Bio-Therapeutics

Over the past 30 years protein bio-therapeutics experienced major clinical successes and nowadays represent the fastest growing category of drugs. Meanwhile, progress in the understanding of RNA biology has led to the identification of new RNA classes with unanticipated functions. Following these advances new therapeutic strategies based on nucleic acids have been introduced. Examples are single stranded antisense oligonucleotides, double stranded small interfering RNAs and microRNAs, all for inhibiting gene expression and, most recently, single guide RNAs for the editing of mutated genes. In addition to nucleic acids which silence or edit genes, there is a major interest as well in the therapeutic potential of messenger RNAs to express proteins in cells for e.g. vaccination to protect the population against infections or to treat cancer patients. As 30-40 years ago, when recombinant proteins and monoclonal antibodies began to be developed as therapeutics, it seems that with nucleic acid bio-therapeutics we are again on the brink of a revolution in drug development. Today the majority of clinically advanced nucleic acids are systemically injected and tested for liver associated diseases. However, numerous potential targets for nucleic acids are present in tissues beyond the liver, like in the lungs and the eyes, which can best be reached through local administration of the nucleic acids, like through inhalation in the lungs or local injections in the eye.